80-O16B

一种可电离的阳离子脂质；已被用于生成脂质纳米颗粒（LNPs），用于在小鼠中递送CRISPR互补sgRNA和Cas9以实现基因组编辑。含有80-O16B与PBA偶联的LNPs能够包裹mRNA报告基因，并在HeLa癌细胞中增加荧光素酶报告基因的表达。此外，含有80-O16B与PBA偶联并包裹p53 mRNA的LNPs能够降低DU145、SiHa和HeLa癌细胞的活性。An ionizable cationic lipidoid; has been used in the generation of LNPs for the delivery of CRISPR complementary sgRNA and Cas9 for genome editing in mice; LNPs containing 80-O16B conjugated to PBA and encapsulating an mRNA reporter increase luciferase reporter expression in HeLa cancer cells; LNPs containing 80-O16B conjugated to PBA and encapsulating p53 mRNA decrease the viability of DU145, SiHa, and HeLa cancer cells.分子式C39H78N2O4S4分子量767.3O=C(OCCSSCCCCCCCCCCCC)CCN(CCC(OCCSSCCCCCCCCCCCC)=O)CCCN(C)C