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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4° C,2 years.
- 保质期:
Store at 4° C,2 years.
- 英文名:
Fluorescein 5-Isothiocyanate,5-Isothiocyanato fluorescein;5-FITC
- 库存:
现货
- 供应商:
爱必信(上海)生物科技有限公司
- CAS号:
3326-32-7
- 规格:
500mg
| 产品描述 | |
| 描述 | Fluorescein isothiocyanate isomer I is an amine-reactive reagent for the FITC labeling of proteins, microsequencing of proteins, and peptides (HPLC). |
| 应用 | A compound for the FITC labeling of proteins |
| 纯度 | 95% |
| 储存/保存方法 | Store at 4° C,2 years. |
| 基本信息 | |
| 别名 | 5-Isothiocyanato fluorescein;5-FITC |
| 外观 | orange powder |
| 可溶性/溶解性 | Soluble in ethanol (20 mg/ml), DMSO (5 mg/ml), methanol, and DMF. |
| MDL | MFCD00005063 |
| PubChem CID | 18730 |
| Beilstein 注册 | 1407295 |
| EC号码 | 222-042-0 |
分子结构图:
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文献和实验Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein
, and a useful modification is to use fluorescein isothiocyanate (FITC)-casein in place of casein in the gels. The FITC-casein system is also more sensitive. In this case, the gels are viewed and photographed with ultraviolet (UV) illumination, and proteinase
Fluorescein Diacetate (FDA ) 1. Place pollen on slide. 2. Incubate in humid chamber (wet Whatman in Tupperware) ~ 30 min. 3. Add drops of FDA (2mg/ml in acetone) to Pollen Growth Media (~/ml
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable




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