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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution.
- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- CAS号:
33985-68-1
- 规格:
50mg/10mg
| 规格: | 50mg | 产品价格: | ¥3450.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥1150.0 |
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抑制剂描述: 产品名称:DIM-C-pPhOCH3 产品别名:见爱必信官网 英文别名:DIM-C-pPhOCH3 靶点:Nur77 CAS:33985-68-1 纯度:98% 外观:见爱必信官网 保存方法:Store at -20℃ for one year(Powder);Store at 2-4℃ for two weeks;Store at -20℃ for six months after dissolution. 描述:DIM-C-pPhOCH3 is a Nur77 agonist. Nerve growth factor-induced Bα (NGFI-Bα, Nur77) is an orphan nuclear receptor. 溶解性:DMSO : ≥ 33.3 mg/mL (94.49 mM) 体外研究: DIM-C-pPhOCH3 decreases survival and induces apoptosis in RKO colon cancer cells, and this is accompanied by induction of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) protein. DIM-C-pPhOCH3 also induces Nur77-independent apoptosis. DIM-C-pPhOCH3 (10 μM) inhibits cell growth after treatment for 24, 48, or 72 h, and the maximum inhibitory response is observed after 72 h, where there is considerable cell detachment and dead cells. The growth-inhibitory effects observed for DIM-C-pPhOCH3 after 72 h are also accompanied by several markers of apoptosis, including PARP cleavage and cleavage of caspase-3, caspase-9, and caspase-8. PARP cleavage is also observed after treatment of RKO cells for 48 h with DIM-C-pPhOCH3. 体内研究:DIM-C-pPhOCH3 (25 mg/kg/d) also inhibits tumor growth in athymic nude mice bearing RKO cell xenografts. The effects of DIM-C-pPhOCH3 (25 mg/kg/d) on colon tumor growth are also investigated in athymic nude mice bearing RKO cell xenografts. Treatment with the DIM-C-pPhOCH3 significantly decreases tumor volumes and final tumor weights compared with corn oil controls.
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文献和实验的 ULTRAhyb-Oligo 缓冲液(17x 缓冲液)。使用前需在 68℃ 环境中进行溶解。 ( 7 ) 用 0.5 U T4 多核苷酸激酶(PNK,Roche) 和 6 μl [γ-32P ] ATP ( 5000 Ci/mmol ) 标记 1 μmol/L DNA 寡核苷酸(约含有 10 个与目的基因互补的 DNA 寡核苷酸)。 ( 8 ) 参照 Hamilton 和 Baulcombe 的方法,在 35°C 条件下进行 RNA 印迹杂交[14]。 3. 注释 注
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