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- 爱必信(absin)
- 上海
- abs45156461
- 2026年03月31日
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5mg
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试剂描述： 产品名称：CDK5 Substrate [PKTPKKAKKL], Biotinylated 产品别名：Biotin - PKTPKKAKKL CAS号： 英文别名：CDK5 Substrate [PKTPKKAKKL], Biotinylated 分子量：1364.8 分子式： 纯度： ≥ 95% 外观：见爱必信官网 保存方法： 描述：The native peptide PKTPKKAKKL is derived from histone H1 peptide sequence that is docked in the active site of cyclin-dependent kinase 5. It is an effective CDK5 substrate (Km = 40 µM). 应用：
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文献和实验Western Blotting with Biotinylated Antibodies
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. Incubation with Biotinylated Primary Antibody Primary antibody 1.Dilute the antibody in the corresponding blocking buffer
Detection of DNA Sequences Using Biotinylated Probes
It was found ( 1 ) that biotinylated analogs of dUTP, which contain a biotin molecule covalently bound to the C-5 position of the pyrimidine via an allylamine linker arm, can be used as substrates for DNA polymerase. Figure 1 shows the structure
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
3:5. Incubate 100 μl/well of biotinylated reporter antibody diluted in PBS for 2 hours at RT covered with platesealer6. Incubate 100 μl/well of streptavidin alkaline phosphatase, 1:5000 dilution in PBS for 1 hour at RT,covered with plate sealer.7. Wash plates
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