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- 爱必信(absin)
- 上海
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- 2026年03月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
store at -20 ℃ for one year.
- 保质期：
12个月
- 英文名：
霍乱毒素B亚基-FITC、CHOLERA TOXIN B SUBUNIT-FITC、CTB-FITC、 Cholera Toxin B subunit-FITC、CTxB-FITC、Choleragenoid-FITC
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- CAS号：
131096-89-4
- 规格：
1mg
| 产品描述 | |
| 描述 |
Cholera toxin （CTB,霍乱毒素B亚基）belongs to the AB5 –subunit family oftoxins.The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits(~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and sub sequent internalization (transmembrane transport)of the enzymatic component. |
| 纯度 | ≥95% |
| 浓度 | See the label for details. |
| 储存/保存方法 | store at -20 ℃ for one year. |
| 技术指标 |
Format：Recombinant Source：E.coli Sodium azide：Not add. |
| 产品用途 | Cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms.In addition,cholera toxin acts as an adjuvant through the stimulation of B lymphocytes. The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic. |
| 注意事项 | The B subunit is non-toxic to cells and possesses no intrinsic adenylate cyclase activity. The cholera toxin B subunit (CTB) attaches to cells by binding to ganglioside GM1.8 CTB has been shown to be a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface), but not for oligodendrocytes or astrocytes. The B subunit has been reported to be an excellent tracer for the study of axonal transport using immunohistochemical methods. Recently it has been widely used as a marker of membrane lipid rafts, which are membrane microdomains enriched with cholestrol and sphingolipids. These lipid rafts have an important role in cell signaling and protein trafficking. |
| 基本信息 | |
| 别名 | 霍乱毒素B亚基-FITC、CHOLERA TOXIN B SUBUNIT-FITC、CTB-FITC、 Cholera Toxin B subunit-FITC、CTxB-FITC、Choleragenoid-FITC |
| 外观 | liquid |
| 参考文献 | |
| 参考文献 | 1. Nedelkoska, L., and Benjamins, J.A., Binding of cholera toxin B subunit: a surface marker for murine microglia but not oligodendrocytes or astrocytes. J. Neurosci. Res., 53, 605-612 (1998). 2. Janes, P.W., et al., Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor. J. Cell Biol., 147, 447-461 (1999). |
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当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
试剂： 1. 0.01mol/L pH7.2 PBS配方。将NaCl 18g、Na2HPO4 1.15g、KH2PO4 0.2g 溶于2000ml三蒸水中，调整pH至7.2； 2. 0.5mol/L pH9.0碳酸盐缓冲液配法。量取0.5mol/L Na2CO3(5.3%)10ml 加入0.5mol/L NaHCO3(4.2%)90ml中，充分混合后，调整pH至9.0； 3. 3%中碳酸钠水溶液的配制。称取1.5g无水正碳酸钠充分溶解于50
试剂： 抗体球蛋白溶液、异硫氰酸荧光素、3%重碳酸钠水溶液、pH8.0的0.01mol/L PBS、1%硫柳汞。 材料： 离心机及离心管、三角烧瓶（25ml）、冰槽、无菌吸管及毛细滴管、透析袋、玻璃棒、棉线及烧杯（500ml）等。 方法与步骤： 1. 抗体的准备。用0—4℃ pH8.0的PBS将免疫球蛋白溶液浓度稀释为30—40mg/ml，置于三角
技术资料
