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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
VCAM-1抗体,
- 抗体英文名：
VCAM1, CD 106, CD106, CD106 Antigen, DKFZp779G2333, INCAM 100, L1CAM, MGC99561, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1, VCAM1_HUMAN. , ,
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- 应用范围：
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- 宿主：
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- 适应物种：
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- 保质期：
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- 抗原来源：
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- 目录编号：
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- 级别：
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- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 标记物：
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- 克隆性：
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- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态：
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- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥2300.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1500.0 |
| 别名 | VCAM-1抗体;VCAM1, CD 106, CD106, CD106 Antigen, DKFZp779G2333, INCAM 100, L1CAM, MGC99561, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1, VCAM1_HUMAN. , , |
| 宿主 | Rabbit |
| 特异性 | VCAM-1 |
| 反应种属 | Human;Mouse; |
| 预测反应种属 | Rat; |
| 应用 | WB=1:500-2000 |
| 分子量 | Predicted molecular weight: 81kDa Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse VCAM-1:701-800/739 |
| 浓度 | 1mg/ml |
| 纯化方法 | affinity purified by Protein A |
| 类型 | Polyclonal Antibody |
| 同种型 | IgG |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 研究领域 | 肿瘤 心血管 细胞生物 细胞粘附分子 内皮细胞 |
| 背景说明 | VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. |
| 细胞定位 | 细胞膜 |
| UniProt | P29533 |
Sample: VCAM1 protein (Human) at 100 ng
Primary: Anti-VCAM-1 (abs119895) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
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Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Polyclonal Antibody Production
Zealand White Rabbits and have them delivered to the ARC. Don’t put all your eggs in one basket only using one rabbit. Let them get accustomed to there home there for two weeks before the first pre-bleed. Pre-bleed
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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