8458 ScienCell 黄嘌呤氧化酶检测试剂盒 XO, Xanthine Oxidase Assay,产品说明: 黄嘌呤氧化酶(XO)主要位于哺乳动物组织的肝脏和肠道中,催化次黄嘌呤羟基化为黄嘌呤,然后羟基化为尿酸和过氧化*。血液中的XO活性通常非常低,肝损伤会导致XO释放到血液中。XO活性或表达可以在痛风和心血管疾病中上调。该比色测定基于XO催化的黄嘌呤氧化,其中形成的过氧化*被过氧化物酶催化并与4-氨基安替比林反应形成产物染料。反应产物在550nm处的颜色强度与样品中的XO活性成正比。 Xanthine Oxidase (XO) located predominantly in the liver and intestine in mammalian tissues and catalyzes the hydroxylation of hypoxanthine to xanthine and then to uric acid and hydrogen peroxide. XO activity is normally very low in blood and liver injury can result in the release of XO into blood. XO activity or expression can be up-regulated in gout and cardiovascular disease. This colorimetric assay is based on XO-catalyzed oxidation of xanthine, in which the formed hydrogen peroxide is catalyzed by peroxidase and reacts with 4-aminoantipyrine to form the product dye. The color intensity of the reaction product at 550nm is directly proportional to XO activity in the sample.