8368 ScienCell NAD/NADH检测试剂盒 NAD, NAD/NADH Assay,产品说明: 烟**腺*呤二核苷酸(NAD)作为蛋白质之间的可溶性电子载体,是参与许多氧化还原反应的重要酶辅因子。NAD通过控制信号传导和转录事件来调节细胞氧化还原状态,使其和相关酶成为各种代谢紊乱的药物靶标。该比色测定基于乳酸脱氢酶催化NAD还原,其中形成的NADH可以将四唑盐INT还原为INT甲臜产物。INT甲臜产物的强度在490nm处表现出最大吸光度,与样品中NADt(NAD和NADH)或NADH的量成正比。该测定对NAD和NADH具有特异性,并且不能检测到NADP或NADPH。 Nicotinamide adenine dinucleotide (NAD), which acts as soluble electron carriers between proteins, is an important enzymatic cofactor involved in many redox reactions. NAD functions in modulating cellular redox status by controlling signaling and transcriptional events, making it and related enzymes drug targets for various metabolic disorders. This colorimetric assay is based on lactate dehydrogenase catalyzed reduction of NAD, in which the formed NADH could reduce tetrazolium salt INT to an INT-formazan product. The intensity of the INT-formazan product, which exhibits an absorbance maximum at 490 nm, is proportional to the amount of NADt (NAD and NADH) or NADH in the sample. This assay is specific for NAD and NADH, and does not detect NADP or NADPH.