乳酸脱氢酶(LDH)是一种存在于大多数真核细胞中的可溶性胞质酶,在细胞因质膜损伤而死亡时释放到培养基中。培养上清液中LDH活性的增加与裂解细胞的数量成正比。ScienCell LDH 细胞*性测定试剂盒提供了一种比色方法,使用含有乳酸、NAD+、隔膜糖和 INT 的反应混合物测量 LDH 活性。 LDH 在 L-乳酸存在下催化 NAD+ 还原为 NADH,而 NADH 的形成可以在偶联反应中测量,其中四唑盐 INT 还原为红色甲臜产物。高度着色和可溶性甲臜的量可以在490nm分光光度法下测量。
Lactate dehydrogenase (LDH), which is a soluble cytosolic enzyme present in most eukaryotic cells, releases into culture medium upon cell death due to damage of plasma membrane. The increase of the LDH activity in culture supernatant is proportional to the number of lysed cells. ScienCell LDH Cytotoxicity Assay kit provides a colorimetric method to measure LDH activity using a reaction cocktails containing lactate, NAD+, diaphrose and INT. LDH catalyses the reduction of NAD+ to NADH in the presence of L-lactate, while the formation of NADH can be measured in a coupled reaction in which the tetrazolium salt INT is reduced to a red formazan product. The amount of the highly colored and soluble formazan can be measured at 490 nm spectrophotometrically.