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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 英文名：
Plate Count Medium, Modified
- 规格：
250mLX4
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文献和实验by centrifugation x 5 min at 600g and resuspend in 5 ml of EPC medium. 6. Cell seeding of slide prior to flow chamber assembly 1) A slide made out of glass, titanium, or other material can be used, and the slide surface can be modified by spin
is optimum) with shaking. Add dimethyl sulfoxide to 10% (70uL). Mix gently. Heat pulse for 15 min. in a 42°C waterbath. Pellet cells in microfuge at 7000rpm, pour off most of sup and plate on appropriate medium. Incubate the plates at 30°C
Preparing 48-Well Plate Feeders
/ml = 38.86 mls Dilution: 38.9 mls (of cell suspension @ 3.5 x 105 cells/ml) + 127.1 mls Media. Take a count at this point, to verify the cell density of 8.2 x 104 cells/ml. Plate-out this feeder media (dilution) into the 48-well plates
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