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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 英文名:
Plate Count Medium with Antibiotic
- 规格:
250mLX4
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文献和实验Preparation of Antibiotic Stock Solutions and Agar Plates
for 4 weeks at 4℃. In fact already after 1 week you can observe reduced selectivity! Therefore I always plate out 25 µl of antibiotic-stock together with bacteria! Picking colonies without wanted content is very annoying.
on the bottom plate of the flow chamber. Make sure that the slide is placed with the cell-seeded side facing up. 4) Pipet 10 ml of warm EPC medium onto the slide. Allow the medium to cover the slide and flow chamber, but do not spill the medium
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
. Resuspend the pellet in Human Feeder Cell Conditioned Medium and count the cells using a hemacytometer. Plate the desired number of cells (approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Human Feeder
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