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文献和实验daydayupmj 最近做了加入刺激因素后细胞AMPK的激活情况,我选择了0,30min ,60min,120min,240min提取蛋白,结果发现在60min 时总AMPK和P-AMPK表达都很弱,有几点不清楚,信号表达会在某个时间点突然降低,然后再回升吗,非磷酸化的蛋白也会随时间变化而变化吗,再有活化情况该如何比较 daydayupmj 还有细胞在加入处理因素前需要同步化处理吗 07yhguan
Activator-Dependent Acetylation of Chromatin Model Systems
1 promoter. Using this system as an example, here we describe the use of a combination of biochemical and biophysical methods to investigate the effect of activator-dependent acetylation on higher order chromatin structure and transcription by RNA polymerase
Transposon Tagging Using Activator (Ac) in Maize
The transposable element Activator (Ac) has been used in several plant species as a tool for gene isolation and characterization. However, it has not been widely utilized in its native host maize, in part, because of a relatively low germinal
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