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文献和实验A Method for Assaying Deubiquitinating Enzymes
for ribosome biogenesis as well as of free Ubs. Deubiquitinating enzymes (DUBs) are known to consist of a large protein family in eukaryotes. For example, the budding yeast has 17 genes for DUBs (4 ). Moreover, so far more than 60 full-length DUB sequences
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
Despite the identification of numerous deubiquitinating enzymes (DUBs) in recent years, the large majority of this class of enzymes has not been well characterized. This chapter describes biochemical methods that can be used to characterize
enzymes (DUBs) reverse the process of ubiquitination, and number nearly 100 in humans. In principle, DUBs represent promising drug targets, as several of the enzymes have been implicated in human diseases. The isopeptidase activity of DUBs
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