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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期：14天
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MedChemExpress LLC
- CAS号：
2182601-72-3
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电询
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Cy5 DBCO chloride
CAS No. : 2182601-72-3
MCE 国际站：Cy5 DBCO chloride
产品活性：Cy5 DBCO chloride 是一种叠氮化物反应探针，DBCO 分子的加入可以通过无铜 “Click Chemistry” 反应对叠氮化物标记的生物分子进行成像。Cy5 DBCO (chloride) 是一种点击化学试剂。它含有 DBCO 基团，可以和含有 Azide 基团的分子发生菌株促进的炔-叠氮环加成反应 (SPAAC)。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: Cy5 DBCO chloride (2-50 μM) can control the extent of labelling by varying the concentration of DBCO-Cy5 in a dose-dependent manner, with the amount of DBCO-Cy5 bound at the cell surface increasing with increasing DBCO-Cy5 concentration.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Incubate the cells at a density of 3×104 cells in a cell culture dish for 3 days and then wash the cells twice with DPBS (pH 7.4). Incubate the cells according to your normal protocol.
2.Incubate the cells with Cy5 DBCO chloride at a final concentration of 20 μM for 1 h at 37°C.
3.After incubation, wash cells with DPBS (pH 7.4) and fix with formaldehyde-glutaraldehyde combination fixative for 15 min at room temperature.
4.After fixation, wash cells twice with DPBS (pH 7.4). Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader.
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文献和实验visualization of either the presence or absence of oligonucleotides in crystals grown from solutions containing both protein and nucleic acid?labeling with the Cy5 dye. In addition to offering the advantage of being able to distinguish between crystals
Site-directed hydroxy radical mapping of nucleosome positions in vitro
). Any high purity grade, low trace metal chemicals should be sufficient. We source ascorbic acid (cat 95209), hydrogen peroxide (cat 95321), sodium chloride (cat 71378) or potassium chloride (cat 60129), and hydrochloric acid (cat 84415) from Fluka
the DNA: BioPrime kit Invitrogen (18094-011)[1] Cy3-dCTP GE Healthcare (PA53021)[2] Cy5-dCTP GE Healthcare (PA55021) [3] Deoxynucleotides (2mM dATP, 2mM dGTP, 2mM dTTP, 0.5mM dCTP mix). Prepared as follows: 4μl 100mM
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