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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2~8℃
- 保质期：
1年
- 英文名：
PEIpro® transfection reagent
- 库存：
50
- 供应商：
北京百奥创新科技有限公司
- 规格：
10 mL
Concentration :1 mg/mL.
Molecule delivered :Plasmid DNA and co-delivery of several plasmid DNA.
Applications :Large-scale virus production.
Virus types :Mainly adenovirus, Adeno-Associated Viruses (AAV), Lentivirus, influenza virus, retrovirus, Virus-like particles (VLP).
Cell types :Mammalian producer cell lines (HEK-293, HEK-293 derivatives, BHK, VERO cell lines, virus-specific packaging cell lines.
Number of transfections :100 mL of PEIpro® transfection reagent is sufficient to transfect on average 50 L of cell culture.
Storage : Store PEIpro® at 5 °C ± 3°C. Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.
Provided with : Protocol. Certificate of Analysis. Certificate of Origin. Non-Hazardous Product Statement. Summary:PEIpro® transfection reagent is the leading chemical-based DNA transfection reagent that offers flexibility and scalability to develop a robust and sustainable Process Development for viral vector production. PEIpro® benefits from extensive research development in PEI polymer chemistry and formulation to achieve highest transfection efficiency in both adherent and suspension cell culture systems. Protocols established with PEIpro® during Process Development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of PEIpro®: PEIpro®-HQ and PEIpro®-GMP.
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文献和实验材料： 质粒DNA 指数生长的真核细胞 PEI （聚乙烯亚胺） 1×HBS （pH7.4） 配方： PEI 储存液（100 μM）：称取125 mg PEI 粉末溶解于50 ml 1×HBS （pH7.4）中， 0.2 μm 滤膜过滤，储存于4℃备用。 1×HBS （pH7.4）： 将8.76 g NaCl 溶解于900 ml 超纯水，加入20 ml 1 M 的HEPES，调pH 值到7.4，定容至1 L，过滤（0.2 μm 滤膜）后储存于4℃备用。 方法
liufengxi 我用PEI（sigma 25kDa）作为转染试剂，但是发现，同样的细胞，同样的N/P 条件下。转染效果一次比一次要差，难道是PEI容易在水性条件下降解？ 我们的这个PEI买的时间比较久了，不知道是不是该买新的了。 一直找不出原因，很是郁闷，请大虾帮忙解答， dafang0212 PEI粉末放置久了后，是会出现转染效率变差的状况的。另外不知道你的PEI用做转染前有进行加热
Topical Gene Therapy for Pulmonary Diseases with PEI-DNA Aerosol Complexes
Different methods for delivery of genes to the lungs such as intravenous injection, and nasal, or intratracheal instillation have been reported in animal models. However, these strategies are invasive or they may not distribute the material
