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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科技有限公司
- 检测范围:
125 - 8,000 ng/mL
- 检测方法:
Colorimetric
- 样本:
Plasma, Serum
- 灵敏度:
89.12 ng/mL
- 规格:
96T
| 产品名 | Emibetuzumab ELISA Kit |
| 货号 | KDC34201 |
| 规格 | 96T |
| 别名 | LA480, LY-2875358, CAS: 1365287-97-3 |
| 应用 | Used for the quantitative determination of Emibetuzumab concentration in serum and plasma. |
| 检测方法 | Colorimetric |
| 样本类型 | Plasma, Serum |
| 实验类型 | Quantitative |
| 检测范围 | 125 - 8,000 ng/mL |
| 灵敏度 | 89.12 ng/mL |
| 回收率 | 80-120% |
| 精准度 | CV<20% |
| 描述 | PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human MET has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Emibetuzumab in the sample competitively binds to the pre-coated protein with biotin-labeled Emibetuzumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Emibetuzumab bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| 背景 | Emibetuzumab (as known as LY2875358) is a humanized immunoglobulin G4 monoclonal bivalent anti-MET antibody that blocks MET signaling. This drug was developed by Eli Lilly & Company and has been used in trials studying the treatment of Advanced Cancer, Renal Cell Carcinoma, Hepatocellular Cancer, Gastric Adenocarcinoma, and Non-Small Cell Lung Cancer, among others. In preclinical models, emibetuzumab inhibited growth of tumors that are dependent on MET, including tumors that exhibit HGF-dependent and HGF-independent biology. Administration of a single dose of emibetuzumab resulted in continuous reduction of MET protein expression for 14 days in mouse xenografts. Emibetuzumab demonstrated dose-dependent, single-agent activity in various MET-expressing tumor models, and additive effects were observed in combination with erlotinib for reducing tumor volume in lung cancer mouse xenograft models. In Phase 1 dose escalation studies, emibetuzumab monotherapy was well tolerated in patients with advanced solid tumors, including gastric cancer, with no dose-limiting toxicities. |
| 稳定性和存储 | When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. |
| 保存方法 | 2-8 ℃ |
| 说明 | For Research Use Only. |

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