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- 详细信息
- 文献和实验
- 技术资料
- 库存:
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- 英文名:
AGED RAT PRIMARY AORTIC ENDOTHELIAL CELLS
- CAS号:
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- 保质期:
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- 供应商:
北京百奥创新科技有限公司
- 保存条件:
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- 规格:
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| Product Name | Aged Rat Primary Aortic Endothelial Cells |
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| Product Code | RAECs |
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| Cat.No | ABC-TC5508 |
Species | Rat |
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| Size/Quantity | 1 vial |
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| Shipping Info | Dry Ice |
Storage | Liquid Nitrogen |
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| Description | Aged Rat Primary Aortic Endothelial Cells from AcceGen are isolated from aorta of 74 week old laboratory Sprague-Dawley rat. Aged Rat Primary Aortic Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×106 cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody. |
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| Application | For research use only |
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| Product Type | Rat Primary Cells |
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| Product Image | ![]() |
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文献和实验正常人脐静脉原代内皮细胞培养一、实验试剂1、培养基: PriCells Medium + 10% FBS + 1% P/S + PriCells Supplem e nt2、冻存液: PriCells Medium + 20% FBS + 10% DMSO3、洗涤液: 1 × PBS (pH 7.4 ) + 1% P/S4、染色液: 0.4% Trypan Blue5、消化液: PriCells Isolation of Primary Cell Kit6、检测试剂:抗人
的[5],但细胞得率均较低,而近些年来国外大多数方法采用以大脑皮质为材料、酶消化及梯度离心分离脑微血管段并进行原代培养[6~9,11,12]。由于原代培养中无法避免地混杂成纤维细胞、周细胞、平滑肌细胞、星型胶质细胞等其他细胞的生长,而且工作量大、过程繁琐且费用高[1],进行较纯的大鼠脑微血管内皮细胞原代培养一直是国内外的一个难点。 本人参照文献[6]、[7]、[8]的方法,成功地摸索出大鼠脑微血管内皮细胞分离和原代培养的方法,并获得纯度较高的脑微血管内皮细胞。 1.材料与方法 1.1 实验动物 每次
酶消化传代。 5、内皮细胞鉴定:相差显微镜下,细胞呈单层铺路石状;免疫组化证明有vWF相关抗原存在。 6、注意事项:将分离出来的主动脉热处理时温度以60℃2秒为宜,太低则成纤维细胞多,太高或时间久则内皮细胞迁出减少。
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