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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Labeled Sphingomyelin Synthesis 合成标记的神经鞘磷脂
, a little methanol may be added to completely dissolve the material.--------------------------------------------------------------------------------Column purification of labeled SM: 12) Pack a Bio-Sil A column as follows: a) Pack base of column
Sphingomyelin Mass Measurement 鞘磷脂质量分析
O/methanolic phase and collect the lower, CHCl3 phase. 19) Dry the lipids down under extra dry nitrogen gas, resuspend them in 75µl of CHCl3 , aliquot 10 µl for phosphate measurements and spot 50µl on a TLC plate. 20) Spot standards (1-100 nmoles SM
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