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- 文献和实验
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- 英文名:
SIGMA
- 库存:
999
- 供应商:
麦飞生物
- CAS号:
9046-28-0
- 规格:
G9637-100UN
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
kidney D-amino-acid oxidase (Sigma, St. Louis, MO or Boehringer Mannheim, Germany) was used as a control. Organs, tissues, or tissue culture cells were homogenized in distilled water and centrifuged at 550 x g for 5 min. The supernatant solutions
准备工作: DF12 加入0.1%BSA,100ml,取10ml冰浴,40ml放入孵箱;其余的放入4度; DNase I 100*; PI:50ug/ml; Hoechst33342: 1000ug/ml(all from Sigma) 计数板,冰盒;各种离心管,无菌流式管(BD),400滤网(BD) 染色方案: ① 细胞染色: 细胞消化计数,用DF12重悬,调整到1×106/ml浓度,分成三组2管; 37℃DF12洗涤,制备成单
Methods for the Detection of D-Amino-Acid Oxidase
kidney D-amino-acid oxidase (Sigma, St. Louis, MO or Boehringer Mannheim, Germany) was used as a control. Organs, tissues, or tissue culture cells were homogenized in distilled water and centrifuged at 550 x g for 5 min. The supernatant solutions
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