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- 普健生物可提供羊驼免疫，纳米抗体VHH噬菌体建库及淘选技术服务，更有千亿级VHH纳米抗体天然噬菌体文库可淘选各类靶点抗原，大大缩短药物开发周期。
- 武汉
- 2025年07月15日
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- 提供商：
普健生物
- 服务名称：
羊驼天然纳米抗体文库定制
- 规格：
抗体药物开发
服务简介：
纳米抗体是由比利时科学家 Hamers 等 1993 年在《Nature》中首次报道，它是一种天然存在的仅由两条重链组成的特殊抗体，只含有重链可变区（VHH, Variable Domain of Heavy Chain Antibody）和两个常规的 CH2 与 CH3 区，该可变区能单独稳定地在体外存在，是目前已知的可稳定结合目标的最小单位，VHH 晶体宽为 2.5nm，长 4nm，分子量仅为传统完整抗体的 1/10（约 15KD）但依然具有完整的抗原识别能力，因此称之为纳米抗体（Nanobody 或 single domain antibody）
纳米抗体特点：
- 有更长的 CDR3 区（纳米抗体 CDR3 通常由 16~24 个氨基酸组成，传统单抗 CDR3 仅有 7~12 个氨基酸），，与抗原特异性结合能力更强
- 结构简单，易于基因工程改造
- 制备简单，易于工业化放大生产
- 易于人源化
普健生物具有近千亿级别的天然纳米抗体库，可提供天然纳米抗体快速筛选服务，最快在 2-3 周内完成针对各类靶蛋白的特异性纳米抗体快速筛选，亲和力达到 10-9M 级别。
- 样本丰富：源自百余只不同种类的驼类样本--羊驼（Alpaca）、骆驼（Camel）、美洲鸵（Llama），提供更高的抗体多样性。
- 库容量大：总库容近 1011pfu，未来将优化到超千亿级别。
- 超高品质：插入正确率 100%，序列正确率 97%，随机选取 200 个克隆测序，均无重复序列。
案例展示
根据文库中数百个随机抗体序列分析，其 CDR3 长度基本符合正态分布，跟文献中报道的自然 VHH 抗体 CDR3 长度分布相符。
靶点 A 蛋白用天然纳米抗体库快筛，经 4 轮淘选后，挑选的 96 个单克隆中有 56 个阳性克隆（差值均大于 0.5），阳性率为 58%
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羊驼天然纳米抗体文库定制（抗体药物开发）
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