Lymphocyte Activation Gene 3 (

【申请上传】Current Protocols in Immunology

as Adjuvants Unit 2.12 Cloning, Expression, and Modification of Antibody V Regions Unit 2.13 Production of Bispecific Antibodies Unit 2.14 Nucleic Acid Immunizations Chapter 3 In Vitro Assays for Mouse Lymphocyte Function Introduction Section I Isolation

流式细胞技术测定NF-kappaB的转移

antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min

利用人工组合转录因子对人类基因组扫描

and their attendant transcription factors provide the spatial-temporal cues that direct when, where, and to what extent a given gene is expressed. Most regulatory sequences contain binding sites for repertoires of transcription factors that mediate activation