- 询价
- Lucigen
- 进口试剂
- 30035-1
- 2025年07月12日
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- 保存条件：
-20º C
- 库存：
100
- 供应商：
远慕生物
- 规格：
500 rxns
美国Lucigen公司主要开发各类用于基因克隆的试剂盒及相关产品，凭借其独到的产品技术，过硬的产品质量，良好的产品服务赢得了全球广大用户的信赖。
Lucigen Corporation is working to improve people’s lives by providing quality products and services to life science and healthcare professionals. Founded in Wisconsin in 1998, Lucigen offers products and services that enable life-science professionals to perform their research and testing more efficiently and effectively. The company has grown to manufacture and sell over 325 biomedical research products and services to customers worldwide, all under an ISO 13485 compliant quality system. Core competencies include enzyme evolution, protein expression, cloning, competent cells, next gen sequencing, and molecular diagnostics. "
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文献和实验Extraction of DNA using DNAzol® Reagent
pipette tips by cutting 2-3 mm from the ends of plastic pipette tips. Mix DNA solutions by inversion; avoid shaking or use of a Vortex for mixing. Homogenization of tissues: Homogenize tissue samples in a hand-held glass/Teflon® homogenizer
SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
实验原理 The system consists of two major components: SuperScript™ III RT/ Platinum® Taq High Fidelity Enzyme Mix and 2X Reaction Mix. SuperScript™ III Reverse Transcriptase is a version of M-MLV RT that has been engineered
充分的准备是实验成功的关键，那么，qPCR 实验需要提前做好哪些了解呢？ 试剂的储存条件 市面上大多数 qPCR 试剂推荐 - 20 ℃ 避光长期储存。 Vazyme 的 ChamQTM 和 AceQ® 两个系列的 qPCR Master Mix，长期储存应置于 - 20 ℃ 避光，解冻后可于 4 ℃ 短暂存放；避免反复冻融。 原始模版稀释倍数 尽量选择 Ct 值落在 15 - 28 范围的稀释倍数作为原始模板稀释度。 反应体系与次数 推荐 20 μl 体系，Vazyme
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