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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
5 - FITC Protein Labeling Kit *Ultra Convenient*
- CAS号：
5 - FITC Protein Labeling Kit *Ultra Convenient*
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
-20°C
- 规格：
1 kit
5 - FITC 蛋白质标记试剂盒荧光法、5 - FITC Protein Labeling Kit *Ultra Convenient*详细如下：
FITC-蛋白偶联物适用于免疫荧光染色、原位杂交、流式细胞术和其他生物学应用。该试剂盒具有进行偶联反应和纯化 FITC 蛋白偶联物的所有必要组件。该试剂盒适用于三个偶联反应，每个反应最多可标记 5 mg IgG。
异硫qing酸荧光素 (FITC) 仍然是最流行的荧光标记试剂之一，这可能是由于该材料的低成本。5-FITC 和 6-FITC 具有非常相似的吸收和荧光光谱。然而，异构体可能在它们与蛋白质的结合和反应性方面有所不同，并且缀合物可能在不同的色谱条件下洗脱或在电泳凝胶中以不同的方式迁移。5-FITC 比 6-FITC 异构体更广泛地用于蛋白质标记。
注意：AnaTag 试剂盒不适用于肽标记。
Add reaction buffer (component B) at 1/10 (v/v) ratio to your target protein (e.g. antibody) solution (2-10 mg/mL is the recommended concentration range of protein).
Note 1: The protein can be dissolved in phosphate, carbonate, borate, triethanolamine or MOPS buffer, pH 7.2-7.5, without reducing reagents (e.g. DTT), protein stabilizers (e.g. BSA) or sodium azide. If the protein is dissolved in Tris or glycine buffer, it should be dialyzed against 0.01 M phosphate buffer saline, pH 7.2-7.4 to get rid of free amines. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed before performing the dye conjugations.
Note 2: The conjugation efficiency is poor when the concentration of protein is less than 2 mg/mL. Meanwhile, the purification column included in this kit can maximally purify 3 mL conjugate solution. You may concentrate the protein solution using a speed vacuum or a centrifugal filter (Millipore, Cat# MRCPRT010).
Add 20 uL of DMSO (component D) to one vial of 5-FITC (component A). This gives a 10 mg/mL of dye solution (26 mM). Completely dissolve all the dye contents by vortexing.
Note: Dye solution should be prepared fresh for each conjugation reaction.Extended storage of the dye solution may reduce dye activity. Any solutions containing the dye must be kept from light.
Note: The procedure given here is optimized for IgG (MW ~ 150,000) labeling with 5-FITC. The dye: protein molar ratio is 12:1. For proteins other than IgG, the optimal dye/protein molar ratio may need to be determined. It will normally be between 2:1 and 20:1.
FITC-蛋白偶联物适用于免疫荧光染色、原位杂交、流式细胞术和其他生物学应用。该试剂盒具有进行偶联反应和纯化 FITC 蛋白偶联物的所有必要组件。该试剂盒适用于三个偶联反应，每个反应最多可标记 5 mg IgG。
异硫qing酸荧光素 (FITC) 仍然是最流行的荧光标记试剂之一，这可能是由于该材料的低成本。5-FITC 和 6-FITC 具有非常相似的吸收和荧光光谱。然而，异构体可能在它们与蛋白质的结合和反应性方面有所不同，并且缀合物可能在不同的色谱条件下洗脱或在电泳凝胶中以不同的方式迁移。5-FITC 比 6-FITC 异构体更广泛地用于蛋白质标记。
注意：AnaTag 试剂盒不适用于肽标记。
Add reaction buffer (component B) at 1/10 (v/v) ratio to your target protein (e.g. antibody) solution (2-10 mg/mL is the recommended concentration range of protein).
Note 1: The protein can be dissolved in phosphate, carbonate, borate, triethanolamine or MOPS buffer, pH 7.2-7.5, without reducing reagents (e.g. DTT), protein stabilizers (e.g. BSA) or sodium azide. If the protein is dissolved in Tris or glycine buffer, it should be dialyzed against 0.01 M phosphate buffer saline, pH 7.2-7.4 to get rid of free amines. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed before performing the dye conjugations.
Note 2: The conjugation efficiency is poor when the concentration of protein is less than 2 mg/mL. Meanwhile, the purification column included in this kit can maximally purify 3 mL conjugate solution. You may concentrate the protein solution using a speed vacuum or a centrifugal filter (Millipore, Cat# MRCPRT010).
Add 20 uL of DMSO (component D) to one vial of 5-FITC (component A). This gives a 10 mg/mL of dye solution (26 mM). Completely dissolve all the dye contents by vortexing.
Note: Dye solution should be prepared fresh for each conjugation reaction.Extended storage of the dye solution may reduce dye activity. Any solutions containing the dye must be kept from light.
Note: The procedure given here is optimized for IgG (MW ~ 150,000) labeling with 5-FITC. The dye: protein molar ratio is 12:1. For proteins other than IgG, the optimal dye/protein molar ratio may need to be determined. It will normally be between 2:1 and 20:1.
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5 - FITC 蛋白质标记试剂盒荧光法
￥4312
