520 组织蛋白酶 S 检测试剂盒 荧光法、520 Cathepsin S Assay Kit *Fluorimetric*详细如下: 组织蛋白酶是一类在哺乳动物细胞更新中发挥重要作用的球状溶酶体蛋白酶。它们降解多肽并通过它们的底物特异性来区分。 520 组织蛋白酶 S 活性检测试剂盒使用 5-FAM/QXL™ 520 标记的 FRET 肽底物来测量酶活性。在完整的 FRET 肽中,5-FAM 的荧光被 QXL™ 520 淬灭。在 FRET 肽被活性酶切割后,可以在激发/发射 = 490 nm/520 nm 下连续监测荧光的增加。基于 5-FAM/QXL™ 520 的 FRET 肽具有出色的荧光量子产率和更长的发射波长,受测试化合物和细胞成分的自发荧光干扰较少,并提供更好的检测灵敏度。检测限:1 ng/ml、主持人:大肠杆菌 Prepare assay buffer: Prepare fresh assay buffer for each experiment according to Table 1. Use this DTT-containing assay buffer in all the following steps. Cathepsin S substrate solution: Dilute Cathepsin S substrate (Component A) 1:100 in assay buffer. For each experiment prepare fresh substrate solution. Cathepsin S diluent: Dilute the enzyme (Component C) 1:400 in assay buffer. This amount of enzyme is enough for a full 96-well plate. If not using the entire plate, adjust the amount of enzyme to be diluted accordingly. Cathepsin S inhibitor (E-64): Dilute the 1 mM inhibitor solution (Component E) 1:100 in assay buffer to get a concentration of 10 uM. Add 10 ul of the diluted compound into each of the inhibitor control well. Add test compounds and diluted enzyme solution to the microplate wells. The suggested volume of enzyme solution for one well of a 96-well plate is 40 uL and test compound is 10 uL.