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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4-25℃
- 保质期：
1 Year
- 英文名：
Solubilization Buffer, DIGE
- 规格：
250mL
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文献和实验Quantifying Protein in 2-D PAGE Solubilization Buffers
or loading extract from the same number of cells is inaccurate. The author has observed that radioactively labeled cell extracts exhibit 10–20% variances in protein concentration. Similarly, solubilization of 2�106 cells from a number of colorectal carcinoma
Solubilization and renaturation of proteins from inclusion bodies
Solubilization and renaturation of proteins from inclusion bodies Materials Wash buffer 50 mM Tris-HCl pH 7.5 50-200 mM NaCl
Protein Extraction from Solid Tissue
Maximal extraction and solubilization of protein from diseased or healthy tissue is important to make the whole protein complement available for proteomic analysis. It also helps to maximize reproducibility and to minimize waste. Minimal
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