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- 2025年10月22日
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- 保存条件：
4-25℃
- 保质期：
1 Year
- 英文名：
T7 Transcription Buffer for DNA TemPlate Preparation (10x)
- 规格：
10mL
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文献和实验or more of highly purified RNA for structural studies by nuclear magnetic resonance (NMR) or X-ray crystallography. Therefore, robust and scalable protocols, such as those described in this chapter, for production of plasmid DNA template, RNA in vitro transcription
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
of culture fluid. When using one of the accompanying sequencing methods, one cycle sequence reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB (+antibiotic) culture with single
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids*
plasmid per ml of culture fluid. When using one of the accompanying sequencing methods, one cycle sequence reaction will require 160-300ng of template. 1. Streak clone stock to single colony on LB (+antibiotic) plate. Inoculate 1ml LB
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