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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Purified anti-DNA-PKcs Phospho (Thr2609) Antibody
- CAS号：
Purified anti-DNA-PKcs Phospho (Thr2609) Antibody
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
25 ug/ 100 ug
| 规格： | 25 ug | 产品价格： | ￥2000.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥5000.0 |
Description：
DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.
Other Names：DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
Isotype：Mouse IgG1, κ
Reactivity：Human, reacts with Thr2609-phosphorylated DNA-PKcs
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：Modified peptide
Formulation：This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation：The antibody was purified by affinity chromatography.
Concentration：0.5 mg/ml
Storage & Handling：Upon receipt, store undiluted between 2°C and 8°C.
Application：ICC - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations：Eid M, et al. 2014. J Immunol. 192:5529. PubMed
Structure：PI3/PI4-Kinases family. Spliced products 460 kD, 470 kD
Distribution：Nuclear
Function：Serine/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator
Interaction：Ku70/Ku80, KIP, DNA-ligase IV, XRCC4
Modification：Phosphorylation
Biology Area：Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family：Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
1. Hartley K, et al. 1995. Cell. 82:849.
2. Connelly M, et al. 1996. Gene. 175:271.
3. Douglas P, et al. 2002. Biochem. J. 368:243
4. Calsou P, et al. 2003. J. Mol. Biol. 326:93.
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选，选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时，又用传统的WB方式来验证这个结果。经过反复验证，都得出了同样的结果， Cell Based
提示我模型中的通路究竟涉及哪个亚型，而我又没有那么多经费一个一个亚型去试，故我想能不能就检测总的PKC激活水平，毕竟有亚型被激活，那总的活化PKC水平也应该会升高啊！虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教：Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同？既然都是pan，那是否说都是针对所有的亚型，只是磷酸化的残基不同
米宝 这俩天做实验，极其郁闷。我做的总AKt和pAkt,大鼠脑组织，第一次跑总AKT的时候，我觉得可能是因为蛋白是提取的浓度比较好吧，出来了一条浓浓的条带，三组总AKt都出了，接下来做pAkt,就出了一个带，效果挺明显的。后来再做pAkt的时候，跑了4张膜，显影的时候，太干净了，上面什么都没有，甚至连个杂带，斑点 都没！实验是在实验员的领导下完成的，技术上基本没什么纰漏，查阅本版面有关这问题方面的介绍，有的解释说pakt降解了！一抗是用的Phospho-Akt
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