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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
TotalSeq™-A0360 anti-human CD357 (GITR) Antibody
- CAS号：
TotalSeq™-A0360 anti-human CD357 (GITR) Antibody
- 保质期：
详见说明
- 保存条件：
2-8℃
- 规格：
10 ug
| 371231-10 | TotalSeq™-B0360 anti-human CD357 (GITR) Antibody | TotalSeq™-B0360 抗人 CD357 (GITR) 抗体 | 10 ug | ￥6,500.00 |
| 371227-10 | TotalSeq™-C0360 anti-human CD357 (GITR) Antibody | TotalSeq™-C0360 抗人 CD357 (GITR) 抗体 | 10 ug | ￥6,500.00 |
| 371233-10 | TotalSeq™-D0360 anti-human CD357 (GITR) Antibody | TotalSeq™-D0360 抗人 CD357 (GITR) 抗体 | 10 ug | ￥6,500.00 |
GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.
Other Names：TNFRSF18, Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR
Isotype：Mouse IgG2a, κ
Isotype Control：TotalSeq™-A0091 Mouse IgG2a, κ isotype Ctrl
Reactivity：Human
Antibody Type：Monoclonal
Host Species：Mouse
Immunogen：Recombinant human GITR-Fc chimera
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application：
PG - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Structure：TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD.
Distribution：Activated lymphocytes.
Function：Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway.
Ligand/Receptor：GITRL
Cell Type：Lymphocytes, Tregs
Biology Area：Costimulatory Molecules, Immunology
Molecular Family：CD Molecules
Antigen References：
1. van der Werf N, et al. 2011. J. Immunol. 187:1411.
2. Shimizu J, et al. 2002. Nat. Immunol. 3:135.
3. McHugh RS, et al. 2002. Immunity 16:311.
4. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
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