ZPCK,26049-94-5

PCR 不必“摸条件”

μL 体系 2× MasterMix 25 μL 上游 Primer （ 10 μM 1-5 μL 下游 Primer （ 10 μM ） 1-5 μL 模板 lamid DNA ～ 50 ng genomic DNA ～ 1000 ng cDNA ～ 500 ng 粗提液 ～ 2 μL 灭菌蒸馏水至 50 μL PCR 扩增实例： 一、plasmid DNA 扩增 1．提取的质粒 DNA 扩增 以pUC19 质粒为模板扩增 100，250，500

Single tube confirmation PCR protocol

concentration of ~10 µM). We use 5 µl of each primer in 50 µl PCR reactions (~ 1 µM final primer concentration). - Label 20 thin-wall PCR tubes (5 for each isolate) and add the following primer pairs: A-B, A-kanB, C-D, kanC-D, and A-D. Tubes 1-5

Focus on PCR

. When using Taq DNA Polymerase,this temperature is typically 94-95°C and lasts 45 seconds to a few minutes. In the second stage, annealing, the primers are annealed to the template. Temperature is critical to the proper attachment of the primer to the single