- ￥1920
- 翌圣生物（Yeasen）
- 20433ES25
- 上海翌圣
- 2026年01月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)
- 保质期：
有效期1年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20℃保存
- 规格：
25µg
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.
产品性质
|
别名(Synonyms) |
UBA1 |
|
来源(Source) |
E.coli |
|
分子量 (Molecular Weight) |
Approximately 118 kDa. |
|
氨基酸序列 (AA Sequence) |
MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYV PVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWL YFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQT WADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHK LEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR |
|
外观 |
Liquid |
|
标签(Tag) |
None |
|
纯度(Purity) |
> 95% by SDS-PAG |
|
缓冲液(Buffer) |
50 mM HEPES, pH8.0, 50mM NaCl. |
储存条件
-25~-15℃保存
注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
反应缓冲液配方
|
名称 |
组分 |
浓度 |
|
10 × Reaction Buffer（200 ul） |
Tris PH8.0 |
500 mM |
|
DTT |
6 mM | |
|
ddH2O |
to 200μL | |
|
10 x ATP-Mg （200 ul）
|
ATP |
100 mM |
|
MgCl2 |
100 mM | |
|
ddH2O |
to 200μL |
参考阳性反应体系
|
组分 |
体积 (μL) |
|
ddH2O |
to 20 μL |
|
10 × Reaction Buffer |
2 μL |
|
10 × E1 Enzyme |
2 μL |
|
5 × Ubiquitin |
4 μL |
|
10 × ATP-Mg |
2 μL |
|
20 × E2 Enzyme |
1 μL |
|
10 × E3 Ligase Itch (Positive control) |
2 μL |
|
20 × S5a substrate (Positive control) |
1 μL |
Ver.CN20250428
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文献和实验Purification of E1 and E1-Like Enzymes
Ubiquitin-activating enzyme is the archetype for a family of enzymes catalyzing the ATP-coupled activation of ubiquitin and other class 1 ubiquitin-like proteins required for their subsequent conjugation to cellular targets. The general
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
酶上形成Ub-E2复合体。随后，E2酶和一些种类不同的E3酶共同识别靶蛋白，对其进行泛素化修饰。根据E3与靶蛋白的相对比例可以将靶蛋白单泛素化修饰和多聚泛素化修饰。 泛素化过程(Stone, Sophia L., 2019) E1：泛素激活酶E1；E2：泛素偶联酶E2；E3：泛素连接酶E3； Target：靶蛋白；Ubiquitin：泛素；DUBs：去泛素化酶。 P.S.单个泛素分子以其C-末端赖氨酸与靶蛋白的一个Lys侧链相连叫做单泛素化，与多个赖氨酸残基相连叫做多泛素化。如果靶蛋白
