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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
100 ug/20 ug
| 规格: | 100 ug | 产品价格: | ¥3080.0 |
|---|---|---|---|
| 规格: | 20 ug | 产品价格: | ¥900.0 |
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文献和实验Purification Scheme for Ubiquitin Expression Lysate
Purification Scheme for Ubiquitin Expression Lysate Step 1: Take the expression lysis supernatent and treat with concentrated acetic acid until the pH drops to between 4.5 and 5.0. The solution will turn milky white
Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
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