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德国美天旎生物技术有限公司
MACSima™全自动空间组图谱成像分析系统
轻松实现同一切片空间多组学成像
轻松实现同一切片空间多组学成像
生物体的正常功能涉及数千种蛋白质的相互作用，生物系统和细胞活动本身就十分复杂。因此，需要检测大量参数来深入分析生物系统，以便了解生物的奥秘。然而，目前可用于检测复杂生物系统的方法非常有限。美天旎基于MICS (MACSima Imaging Cyclic Staining)循环染色成像技术创新性地使用荧光显微技术，可对同一组织切片分析200多个蛋白质标志物和27种RNA。高度自动化的MACSima成像分析系统是美天旎MACSima空间生物学平台的核心。科学家们可借助该系统评估多种不同标志物的定位、表达以及可能存在的相互作用，充分挖掘空间生物学的潜力。
MICS技术⸺基本原理
美天旎的MICS技术基于荧光显微术可同时分析单个样本中的数百种标志物。通过不同荧光标记抗体的循环染色，在不损伤样本的情况下，获取多种参数的显微成像数据。循环染色包括三个主要步骤：荧光染色、图像采集和荧光信号擦除，均由MACSima成像系统全自动完成。最终生成的数百种标志物的空间组图谱可帮助科学家以的深度洞察样本的生理学或病理学特性。该系统具有即时处理能力，即便是在循环染色过程中也可随时开始数据分析。
美天旎的MICS技术基于荧光显微术可同时分析单个样本中的数百种标志物。通过不同荧光标记抗体的循环染色，在不损伤样本的情况下，获取多种参数的显微成像数据。循环染色包括三个主要步骤：荧光染色、图像采集和荧光信号擦除，均由MACSima成像系统全自动完成。最终生成的数百种标志物的空间组图谱可帮助科学家以的深度洞察样本的生理学或病理学特性。该系统具有即时处理能力，即便是在循环染色过程中也可随时开始数据分析。
完整的解决方案
MACSima成像系统采用全自动流程和优化的组件，可极大地简化生成复杂的空间组图谱数据的过程，其优点不言而喻。
- 同一个样本分析数百种标志物
获取空间组图谱数据，对单个样本中的数百种蛋白质及其它抗原进行分析。
- 高度自动化的设备
完成实验设计后即可交由MACSima成像系统自动运行。MACSima成像系统是该平台的核心，利用这台仪器可以实现全自动的循环染色和数据处理。
- 已验证的大量抗体组合
基于大量重组工程的荧光素偶联抗体，支持分析您样本上的数百个标记物，这些抗体专门针对MICS进行了验证。
两种信号擦除机制：使用荧光染料偶联抗体染色 (01) 并对染色样本进行图像采集后 (02)，可利用下列两种机制之一擦除荧光信号。
- 轻松的实验准备
得益于多种即用型 REAscreen™ 抗体Panel，其中包含来自美天旎生物技术的预定义抗体组，可轻松进行全面的分析。使用我们的 MACSwell™ 样品载样架和各种灵活形式的抗体可分析任何类型的固定样品。
- 化繁为简的分析软件
使用 MACS® iQ View 轻松全面地分析您的超高内涵成像数据。其多功能性和直观的用户界面使这款图像分析软件成为您空间生物学实验的完美伴侣。
MACSwell样本载样架
可分析各种固定样本
要想解决复杂的科学问题，必须突破技术限制。为了能够在MACSima成像系统上不受限制地灵活分析各种固定样本，美天旎开发出MACSwell样本载样架。我们针对组织、贴壁细胞和悬浮细胞设计出三种不同类型的载样架。每种载样架都有明确定义的反应腔室，可以轻松且安全地实现循环染色，确保顺利完成实验。
经过验证的固定方法
一次实验、众多结果
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文献和实验该产品被引用文献
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相关实验
图像。以任一种荧光染料或红外染料染色的凝胶，也能获得漂亮的图像。PXi可容纳的凝胶大小在10cm x 12cm以内。 PXi成像仪还附带GeneTools软件。它可在数秒钟之内自动生成结果，包括泳道分析、分子 量及数量计算，帮您节省宝贵的时间。 此外，Syngene近日还推出了安全且灵敏的UltraSafe Blue™荧光染料，在琼脂 糖和聚丙烯酰胺凝胶染色时可取代EB。与UltraSlim LED蓝光透射仪一起使用，UltraSafe Blue可实现0.5pg DNA的检测
膜的完美图像。以任一种荧光染料或红外染料染色的凝胶，也能获得漂亮的图像。PXi可容纳的凝胶大小在10cm x 12cm以内。 PXi成像仪还附带GeneTools软件 。它可在数秒钟之内自动生成结果，包括泳道分析、分子量及数量计算，帮您节省宝贵的时间。 此外，Syngene近日还推出了安全且灵敏的UltraSafe Blue™荧光染料，在琼脂 糖和聚丙烯酰胺凝胶染色时可取代EB。与UltraSlim LED蓝光透射仪一起使用，UltraSafe Blue可实现0.5pg
的演变，以及 TME 中细胞间的相互作用和空间分布仍然知之甚少。因此，本研究旨在利用空间多组学技术，深入剖析人源和鼠源 LUAD 前体样本，解析 TME 的空间结构、细胞组成和功能动态，并寻找潜在的早期干预靶点。 研究路线 研究结论 图 4. 成像质谱细胞术技术绘制的空间免疫微环境图 研究团队收集了 114 例人类 LUAD 及其前体样本，利用成像质谱细胞术（IMC）技术，绘制了高分辨率的空间免疫微环境图谱。研究发现，肺腺癌从癌前病变向浸润性癌转变过程中，伴随着巨噬细胞的极化
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MACSima™全自动空间组图谱成像分析系统
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