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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
HA; HA tag; HA-tag
- 浓度:
Affinity purification
- 宿主:
Mouse
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 保存条件:
Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 形态:
形式:Liquid 储存溶液:Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- 亚型:
IgG
- 免疫原:
A synthetic peptide corresponding to HA tag.
- 规格:
50 µl/500 µl/1000 µl
| 规格: | 50 µl | 产品价格: | ¥630.0 |
|---|---|---|---|
| 规格: | 500 µl | 产品价格: | ¥2685.0 |
| 规格: | 1000 µl | 产品价格: | ¥4410.0 |
稀释方法:IP 1:50 - 1:200
总结:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.
Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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