- ￥4800
- Tanda
- 美国
- TD-26084
- 2025年10月27日
- ELISA, WB, IHC, IF
- Mouse
- 脊椎动物
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- 详细信息
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H.
- 亚型：
IgG
- 形态：
液体
- 保存条件：
零下20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26084
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC, IF
- 浓度：
1mg/ml
- 靶点：
R172H
- 抗体英文名：
Anti IDH2(R172H) Mouse Monoclonal Antibody
- 抗体名：
IDH2(R172H)
- 规格：
100μl
IDH2(R172H)
| Cat. #:: TD-26084 |
| Gene Symbol: IDH2 |
| Description: Anti-IDH2(R172H) Mouse Monoclonal Antibody |
| Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-172 in IDH2 to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
| Immunogen: A synthetic peptide from the internal region of human IDH2 which includes the mutation of R172H. |
| Applications: ELISA, WB, IHC, IF |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 IF: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-IDH2(R172H) antibody recognizes IDH2(R172H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
| Western blot:
Western blot analysis of recombinant IDH2(R172H) and wild type IDH2 proteins.
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172H) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172H) plasmid (right column), then fixed and stained with Anti-IDH2(R172H) monoclonal antibody (Cat. #TD-26084). |
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