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2 racks for use with 2 ml microcentrifuge tubes on the TissueLyser II
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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)
实验步骤 1. Place a empty, uncapped 50 ml conical tube into a 50ml tube holder. 2. Add 1-6 ml blood sample to 50ml tube. 3. Add indicated volume of PBS, MSL, Proteinase K and RnaseA showed in the table to the sample. Mix
He Tube Mating with MSCV recipient
the dilution with the OD closest to 0.1 (this is true for recipient AND donor cultures. You want an actively growing culture for optimal mating) 5. To set up tube mating, you want a 1:1 ratio of recipient:donor. Determine which dilution of the MSCV dilutions
Tube Formation: an In Vitro Matrigel Angiogenesis Assay
according to their specific needs. This chapter describes an easy-to-perform assay that is based on the differentiation of endothelial cells and the formation of tube-like structures on an extracellular matrix, Matrigel. The assay can be used to screen compounds
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