SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
CAS号:
无
规格:
100 µg
重组新冠病毒 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD 蛋白 (His标签)(产品说明)
蛋白名称:SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (His Tag)
蛋白构建:A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529 sublineage BA.2).
表达宿主:HEK293 Cells
蛋白纯度: > 95 % as determined by SDS-PAGE
> 95 % as determined by SEC-HPLC
蛋白活性:Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (His Tag)(Cat:40592-V08H123), the EC50 is 5-30ng/mL.
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (His Tag)(Cat:40592-V08H123), the EC50 is 5-30ng/mL.
Load ACE2,mFc tag(Cat: 10108-H05H)on proA biosensor,can bind SARS-CoV-2 B.1.1.529 sublineage BA.2 (Omicron) Spike RBD Protein (Cat: 40592-V08H123) with an affinity constant of 2.3 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Arg 319
蛋白分子量:The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.71 kDa. As a result of glycosylation, it migrates as an approximately 36.55 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:YP_009724390.1
蛋白氨基酸序列:Arg319-Phe541
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.