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- ATCC
- 30-2005
- 2025年09月30日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
上海觅拓生物科技有限公司
- 规格：
500ML
- Product category
- Media
- Formulation
- Contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in a 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
- Comments
- NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
- Applications
-
Cell culture
Cell growth and viability - Volume
- 500 mL
- Storage conditions
- 2°C to 8°C
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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
实验试剂 Dulbecco’s Modified Eagle Medium (D-MEM) Fetal Bovine Serum (FBS), ESC-Qualified D-MEM/F-12 (1X) with GlutaMAX™-I KnockOut™ Serum Replacement (KSR) MEM Non-Essential Amino Acids Solution, 10 mM
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
clones when thay occupy 25% of the bottom surface of the well.二、SOLUTIONS1、IMDM (Iscove's Modified Dulbecco's Medium with 25 mM Hepes/with L-Glutamine) BioWhittaker Europe Cat.: BE12-722F2、IMDM-FCS(10%v/v)-HTx100(1%v/v)-AJ(5%v/v)-IL-6 (100 U/ml)3、IMDM
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