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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Mouse F(ab')2 IgG1-LE/AF
- CAS号:
Mouse F(ab')2 IgG1-LE/AF
- 保质期:
1年
- 供应商:
齐源生物
- 保存条件:
2-8℃
- 规格:
0.5mg
低内毒素/无叠氮化物小鼠 F(ab') 2 IgG 1,用作流式细胞术、免疫组织化学和体外测定的对照,并用作 ELISA 的标准。
克隆:15H6
同型:小鼠 F(ab') 2 IgG 1 κ
特异性:T-2霉菌毒素
共轭:LE/AF(低内毒素/无叠氮化物)
缓冲液配方:磷酸盐缓冲盐水,pH 7.4
克隆性:单克隆
浓度:0.5 毫克/毫升
体积:1.0 毫升
推荐存储:2-8℃;在无菌条件下处理
应用
流式细胞术 - 质量测试1
ELISA - 质量测试
FLISA - 质量测试
免疫组织化学-石蜡切片 - 文献报道2,3
体外对照 - 文献报道4
Citations
1. Lund CV, Popkov M, Magnenat L, Barbas CF 3rd. Zinc finger transcription factors designed for bispecific coregulation of ErbB2 and ErbB3 receptors: insights into ErbB receptor biology. Mol Cell Biol. 2005;25:9082-91. (FC)
2. Chatelais L, Jamin A, Gras-Le Guen C, Lallès J, Le Huërou-Luron I, Boudry G. The level of protein in milk formula modifies ileal sensitivity to LPS later in life in a piglet model. PLoS One. 2011;6(5):e19594. (IHC-PS)
3. Boudry G, Jamin A, Chatelais L, Gras-Le Guen C, Michel C, Le Huërou-Luron I. Dietary protein excess during neonatal life alters colonic microbiota and mucosal response to inflammatory mediators later in life in female pigs. J Nutr. 2013;143:1225-32. (IHC-PS)
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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
. Birling, M.C., Gofflot, F., and Warot, X. 2009. Site‐specific recombinases for manipulation of the mouse genome. Methods Mol. Biol. 561:245‐263.
Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
Immunohistochemistry: Fluorescence Protocol 2.0
temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 , IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5% human AB serum. Cells are ready for analysis by flow cytometry
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