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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Goat IgG-AF647
- CAS号：
Goat IgG-AF647
- 保质期：
1年
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
100tests
山羊 IgG-AF647、Goat IgG-AF647详细如下：
山羊 IgG-Alexa Fluor ® 647 用作流式细胞术和免疫组织化学/免疫细胞化学测定的对照。
同型：山羊IgG
来源：普通山羊血清
共轭：AF647 (Alexa Fluor® 647 )
缓冲液配方：含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水
克隆性：多克隆
浓度：特定批次
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下
应用
相关格式的质量测试应用包括 -
流式细胞术1-4
ELISA
FLISA
相关格式的其他参考应用包括 -
免疫组化-冷冻切片5-7
免疫组化-石蜡切片8
免疫组化-Whole Mount 18
免疫细胞化学19,20
免疫沉淀21
封闭4,9 ,10
体外对照11-15
体内对照16,17
Citations
1. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-39. (FC)
2. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: "positive" selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
3. Milpied P, Renard A, Bruneau J, Mendes-da-Cruz DA, Jacquelin S, Asnafi V, et al. Neuropilin-1 is not a marker of human Foxp3+ Treg. Eur J Immunol. 2009;39:1466-71. (FC)
4. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (FC, Block)
5. Sleeman MA, Watson JD, Murison JG. Neonatal murine epidermal cells express a functional multidrug-resistant pump. J Invest Dermatol. 2000;115:19-23. (IHC-FS)
6. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)
7. Menke J, Zeller GC, Kikawada E, Means TK, Huang XR, Lan HY, et al. CXCL9, but not CXCL10, promotes CXCR3-dependent immune-mediated kidney disease. J Am Soc Nephrol. 2008;19:1177-89. (IHC-FS)
8. Tamura M, Aizawa R, Hori M, Ozaki H. Progressive renal dysfunction and macrophage infiltration in interstitial fibrosis in an adenine-induced tubulointerstitial nephritis mouse model. Histochem Cell Biol. 2009;131:483-90. (IHC-PS)
9. Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, et al. Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin. Cell Microbiol. 2006;8:823-36. (Block)
10. Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)
11. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (In vitro control)
12. Sato H, Wang D, Kudo A. Dissociation of Pax-5 from KI and KII sites during κ-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. J Immunol. 2001;166:6704-10. (In vitro control)
13. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (In vitro control)
14. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20. (In vitro control)
15. Li H, Usas A, Poddar M, Chen C, Thompson S, Ahani B, et al. Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness. PLoS One. 2013;8(6):e64923. (In vitro control)
16. Lo JC, Chin RK, Lee Y, Kang H, Wang Y, Weinstock JV, et al. Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest. 2003;112:1495-505. (In vivo control)
17. Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1β. Genes Dev. 2006;20:2527-38. (In vivo control)
18. Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)
19. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
20. Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC)
21. Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. (IP)
山羊 IgG-Alexa Fluor ® 647 用作流式细胞术和免疫组织化学/免疫细胞化学测定的对照。
同型：山羊IgG
来源：普通山羊血清
共轭：AF647 (Alexa Fluor® 647 )
缓冲液配方：含有 < 0.1% 叠氮hua钠的磷酸盐缓冲盐水
克隆性：多克隆
浓度：特定批次
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下
应用
相关格式的质量测试应用包括 -
流式细胞术1-4
ELISA
FLISA
相关格式的其他参考应用包括 -
免疫组化-冷冻切片5-7
免疫组化-石蜡切片8
免疫组化-Whole Mount 18
免疫细胞化学19,20
免疫沉淀21
封闭4,9 ,10
体外对照11-15
体内对照16,17
Citations
1. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-39. (FC)
2. Pospisil R, Young-Cooper GO, Mage RG. Preferential expansion and survival of B lymphocytes based on VH framework 1 and framework 3 expression: "positive" selection in appendix of normal and VH-mutant rabbits. Proc Natl Acad Sci USA. 1995;92:6961-5. (FC)
3. Milpied P, Renard A, Bruneau J, Mendes-da-Cruz DA, Jacquelin S, Asnafi V, et al. Neuropilin-1 is not a marker of human Foxp3+ Treg. Eur J Immunol. 2009;39:1466-71. (FC)
4. Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, et al. A pathobiological role of the insulin receptor in chronic lymphocytic leukemia. Clin Cancer Res. 2011;17:2679-92. (FC, Block)
5. Sleeman MA, Watson JD, Murison JG. Neonatal murine epidermal cells express a functional multidrug-resistant pump. J Invest Dermatol. 2000;115:19-23. (IHC-FS)
6. Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, et al. Phenotype, localization, and mechanism of suppression of CD4+CD25+ human thymocytes. J Exp Med. 2002;196:379-87. (IHC-FS)
7. Menke J, Zeller GC, Kikawada E, Means TK, Huang XR, Lan HY, et al. CXCL9, but not CXCL10, promotes CXCR3-dependent immune-mediated kidney disease. J Am Soc Nephrol. 2008;19:1177-89. (IHC-FS)
8. Tamura M, Aizawa R, Hori M, Ozaki H. Progressive renal dysfunction and macrophage infiltration in interstitial fibrosis in an adenine-induced tubulointerstitial nephritis mouse model. Histochem Cell Biol. 2009;131:483-90. (IHC-PS)
9. Boesze-Battaglia K, Besack D, McKay T, Zekavat A, Otis L, Jordan-Sciutto K, et al. Cholesterol-rich membrane microdomains mediate cell cycle arrest induced by Actinobacillus actinomycetemcomitans cytolethal-distending toxin. Cell Microbiol. 2006;8:823-36. (Block)
10. Pepin AC, Tandon R, Cattori V, Niederer E, Riond B, Willi B, et al. Cellular segregation of feline leukemia provirus and viral RNA in leukocyte subsets of long-term experimentally infected cats. Virus Res. 2007;127:9-16. (Block)
11. Mierke CT, Ballmaier M, Werner U, Manns MP, Welte K, Bischoff SC. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med. 2000;192:801-11. (In vitro control)
12. Sato H, Wang D, Kudo A. Dissociation of Pax-5 from KI and KII sites during κ-chain gene rearrangement correlates with its association with the underphosphorylated form of retinoblastoma. J Immunol. 2001;166:6704-10. (In vitro control)
13. Petlickovski A, Laurenti L, Li X, Marietti S, Chiusolo P, Sica S, et al. Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells. Blood. 2005;105:4820-7. (In vitro control)
14. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. J Immunol. 2012;189:3508-20. (In vitro control)
15. Li H, Usas A, Poddar M, Chen C, Thompson S, Ahani B, et al. Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness. PLoS One. 2013;8(6):e64923. (In vitro control)
16. Lo JC, Chin RK, Lee Y, Kang H, Wang Y, Weinstock JV, et al. Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues. J Clin Invest. 2003;112:1495-505. (In vivo control)
17. Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1β. Genes Dev. 2006;20:2527-38. (In vivo control)
18. Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, et al. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature. 2013;498:371-5. (IHC-WM)
19. Hauser SP, Waldron JA, Upuda KB, Lipschitz DA. Morphological characterization of stromal cell types in hematopoietically active long-term murine bone marrow cultures. J Histochem Cytochem. 1995;43:371-9. (ICC)
20. Hsieh-Bonassera ND, Wu I, Lin JK, Schumacher BL, Chen AC, Masuda K, et al. Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering. Tissue Eng Part A. 2009;15:3513-23. (ICC)
21. Kishimoto N, Onitsuka A, Kido K, Takamune N, Shoji S, Misumi S. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection. Retrovirology. 2012;9:107. (IP)
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文献和实验相关实验
的耐光性和良好的荧光量子产量著称。因此很多Alexa Fluor® 染料具有罗丹明核心结构，但是，传统罗丹明的化学结构很难从长波长的荧光染料延伸至远红外区域甚至是更具挑战性的近红外区域，而且生物偶联后其水溶性并不理想。Biotium 科学家发现从绿色到近红外多色荧光的罗丹明染料的新型化学方法。该方法被有效的应用于CF染料的产品中，尤其是远红外CF染料，而且通过这种方法制备的染料不仅水溶性极佳而且耐光性极好。图1. CF系列染料的稳定性，图示为CF633在5min中仍然具有稳定的荧光强度；而AF647
Immune Response Biomarker Profiling
实验试剂 ProtoArray® Human Protein Microarray v5.0 Blocking Buffer and Washing Buffer 10 × Synthetic Block [ 详细信息： Invitrogen ] Alexa Fluor® 647 Goat Anti-Human IgG [ 详细信息： Invitrogen ]
Antibody250µlIF-IC HCA4410SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4412SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F4413SAnti-rabbit IgG (H+L
技术资料暂无技术资料 索取技术资料
山羊 IgG-AF647、Goat IgG-AF647
￥1760
