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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C/-80°C
- 保质期:
12个月
- 英文名:
Recombinant Human Ephrin type-A receptor 3(EPHA3)
- 库存:
4
- 供应商:
上海禾午生物科技有限公司
- 规格:
20ug/100ug/1mg
产品详情
纯度:Greater than 95% as determined by SDS-PAGE.
内毒素:Less than 1.0 EU/ug as determined by LAL method.
生物活性:①Measured by its binding ability in a functional ELISA. Immobilized EPHA3 at 2μg/ml can bind human EFNA5, the EC50 of the protein is 0.9734-1.179 ng/ml.②Human EPHA3 protein his tag captured on COOH chip can bind Human EFNA5 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay.
基因名:EPHA3
Uniprot No.:P29320
别名:AW492086; Cek4; EC 2.7.10.1; EK4; End3; Eph receptor A3; EPH-like kinase 4; EPH-like tyrosine kinase 1; EPHA3; EPHA3_HUMAN; Ephrin receptor EphA3; Ephrin type-A receptor 3; ETK 1; ETK; ETK1; HEK 4; HEK; HEK4; Human embryo kinase 1; Human embryo kinase; Mek4; MGC109882; Receptor tyrosine kinase HEK; Testicular tissue protein Li 64; Tyro 4; Tyro4; TYRO4 protein tyrosine kinase; Tyrosine protein kinase receptor ETK 1; Tyrosine-protein kinase receptor ETK1; Tyrosine-protein kinase TYRO4
种属:Homo sapiens (Human)
蛋白长度:Partial
来源:Mammalian cell
分子量:61.0 kDa
表达区域:21-541aa
蛋白标签:C-terminal 6xHis-tagged
产品提供形式:Liquid or Lyophilized powder
产品描述:人Ephrin Type-A receptor 3 (EPHA3)是一种膜酪氨酸激酶受体,与Ephrin家族配体混杂结合。EPHA3参与迁移和细胞黏附过程,从而介导间质转化和黏附灶组装。所以它是一种已知的结直肠癌致癌基因。该重组蛋白在哺乳动物细胞中表达,并与c端6xHis-tag融合。表达区域为人EPHA3蛋白的21-541aa,分子量为61 kDa。SDS-PAGE测定,最终产物纯度大于95%。用ELISA和LSPR检测重组蛋白的生物物理参数。在功能ELISA中,重组蛋白与人EFNA5的EC50为0.9734-1.179 ng/ml。LSPR测定其与人EFNA5的亲和力常数为13.8 nM。该蛋白产品可用于ELISA和表面等离子体共振,以确定其配体在样品中的存在和浓度,并作为配体结合试验的对照。也可作为免疫原免疫兔或其他动物获得相应的抗体,应用于免疫反应途径相关的免疫学研究。最终产物内毒素含量较低,LAL法测定内毒素含量小于1.0 EU/µg。
更多活性蛋白请浏览上海禾午生物科技有限公司
上海禾午生物科技有限公司 提供重组蛋白生产服务His、GST、MBP等多种表达方式同时进行,确保获得蛋白
蛋白表达与纯化服务 纯化的蛋白经过ELISA,Western-blot,或者其它生物活性验证标签切除测序报告
上海禾午生物科技有限公司是一家生物高新技术企业,产品覆盖分子生物学、细胞生物学、生物学试剂、标准物质,等领域。以研发、销售为核心致力于生命科学产品。已与全国各大科研院校、医院、企业以及国外一些科研机构有良好的合作关系。
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