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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
for counting. Live cells are clear while dead cells are blue. Expect approximately 3 million cells per rat pup, with a viability of approximately 80-90%. 5. Casting fibrin gel constructs - preparation for creating fibrin gels (done well in advance)
v468 Chapter 9 自身启动子报告基因验证转录靶点
Spin Miniprep Kit (Qiagen). 7. Agarose (e.g. from SERVA Electrophoresis, Heidelberg,Germany). 8. 5× TBE-buffer: 54 g Tris base, 27.5 g boric acid, 3.72g EDTA-Na 2 -salt, and ddH 2 O to 1 L (5) , for gel electrophoresis,prepare a 1:10 dilution
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