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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 英文名：
UV Cleavable Biotin-Azide
- 保质期：
2年
- 供应商：
靶点科技
- 保存条件：
-20
- 规格：
10mg
The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
- Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
- Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
- Also available: 13C6-UV-Biotin-azide
产品参数：
反应原理示意图：
1. Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman, K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.; Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
2. An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-Derived Electrophiles by Streptavidin Catch and Photo-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.; Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
3. Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC. Global, in situ, site-specific analysis of protein S-sulfenylation. Nat Protoc. 2015 Jul;10(7):1022-37. View Article
4. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article
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文献和实验Genetic Engineering and Chemical Conjugation of Potato Virus X
compounds. We present three methods of chemical bioconjugation. For direct conjugation of small chemical modifiers to solvent exposed lysines, N -hydroxysuccinimide chemistry can be applied. Bio-orthogonal reactions such as copper-catalyzed azide–alkyne
。Triton-X-100抽提Frizzled-4效果很差，相反，TM-PEK试剂A得到的Frizzled-4在western blot中获得了明显的信号。而TM-PEK试剂对CELSR-3的抽提效果非常令人惊喜，这种全长的358kD蛋白只有TM-PEK试剂A才能获得，而SDS或Triton X-100效果都使人失望。 2． Thermo旗下Pierce的膜蛋白分离试剂盒（货号89881）——该试剂盒首先采用biotin标记膜蛋白（该膜蛋白一般需要有暴露在膜外的赖氨酸残基和足够长度的膜外
UltraLink Immobilized NeutrAvidin (1000‐Å pore size; 50‐ to 80‐µm particle size; 0.08 µmol biotin/ml gel binding capacity; Pierce), prepared as 50% (v/v) slurry containing 0.02% (w/v) sodium azide
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