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100
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上海觅拓生物科技有限公司
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文献和实验MINICHROMOSOME MICROTUBULE BINDING ASSAY
Determine the OD600 and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105 , 1x105 , and 3.0x105 cells/mL GROWTH OF CELLS Grow 100mL of cells to OD600 =0.7-0.8 at 23o C
Minichromosome-Microtubule Binding Assay 微染色体-微管结合实验
with Buffer 1, 15 min. each at 65o C and 2X with Buffer 2, 15min. each at 65o C. Monitor the blot - the last wash may not be necessary. NOTE: Use the 0.9kb Sma1/Pst1 URA3 fragment from pDK377 to visualize pDK370 or other URA3-containing plasmids
微染色体-微管结合实验(Minichromosome-Microtubule Binding Assay)
overnight (at least 18h.). Wash blot 2X with Buffer 1, 15 min. each at 65 o C and 2X with Buffer 2, 15min. each at 65 o C. Monitor the blot - the last wash may not be necessary. NOTE: Use the 0.9kb Sma1/Pst1 URA3 fragment
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