目录号:E365-01B
产品描述:
CRISPR/Cas9是细菌和古细菌在长期演化过程中形成的一种适应性免疫防御系统。CRISPR/Cas9系统通过将入侵噬菌体和质粒 DNA的片段整合到CRISPR序列中,并利用相应的CRISPR RNAs (crRNAs)来指导Cas9蛋白对同源序列的降解,从而提供免疫性。 人工改造过的Cas9/sgRNA系统通过sgRNA(short guide RNA)引导 Cas9蛋白识别并剪切带有sgRNA靶点的双链DNA,可用于基因敲除和精确编辑DNA等操作。本试剂盒提供的Cas9核酸内切酶通过在蛋白两端加了核定位信号(NLS),可使蛋白进入细胞核内进行基因组编辑。
产品特点:
纯蛋白体系,避免CRISPR基因敲除时引入质粒或病毒干扰。
产品用途:
基于蛋白与sgRNA转染的非病毒载体CRISPR基因敲除;
基于蛋白与sgRNA转染的非病毒载体CRISPR基因敲入;
sgRNA剪切靶点DNA效率检测,降低体内筛选成本;
体外剪切靶DNA的特异位点。
保存温度:
-20℃保存。
应用实例:
图例)Cas9 Nuclease 做DNA 片段切割活性检测。
带有靶位点的DNA片段为6100bp;
酶切后的片段为3900bp和2200bp。
参考文献:
Targeting herpes simplex virus with CRISPR-Cas9 cures herpetic stromal keratitis in mice[J]. Di Yin. et al. nature biotechnology. 2021.
Chromatin architecture reveals cell type-specific target genes for kidney disease risk variants[J]. Aiping Duan. et al. BMC Biology. 2021.
Extracellular vesicles engineered with valency-controlled DNA nanostructures deliver CRISPR/Cas9 system for gene therapy[J]. Jialang Zhuang. et al. Nucleic Acids Research. 2020.
Different lineage contexts direct common pro-neural factors to specify distinct retinal cell subtypes[J]. Mei Wang. et al. Journal of Cell Biology. 2020.
Structural insights into DNA cleavage activation of CRISPR-Cas9 system[J]. Cong Huai et al. Nature Communications. 2017.
Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency[J]. Ying Dang et al. Genome Biology. 2015.
High-Effective and Low-Cost microRNA Detection with CRISPR-Cas9[J]. Xin-yuan Qiu et al. ACS Synthetic Biology. 2018.
Efficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents[J]. Gen Zou. et al. microbial biotechnology. 2020.
CRISPR/Cas9-mediated somatic and germline gene correction to restore hemostasis in hemophilia B mice[J]. Cong Huai et al. Human Genetics. 2017.
Pyroptosis Mediates Neutrophil Extracellular Trap Formation during Bacterial Infection in Zebrafish[J]. Weijie Chen. et al. The Journal of Immunology. 2021.
For research use only.