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文献和实验A quick method to isolate plant genomic DNA
the silica pellet with 800 ul wash solution (from the GeneClean II kit). Repeat the wash two times. Dry the pellet (with bound DNA). Resuspend the pellet in 50 ul distilled water. Incubate at 50-65 C for 5 minutes. Spin down pellet
A quick method to isolate plant
down the silica particles (glass milk),5 sec.,remove supernatant.(The DNA in the solution will now hopefully be bound to the silica particles). Wash the silica pellet with 800μl wash solution (from the GeneClean II kit). Repeat the wash
Mag-Binds Plant DNA Kit Magnetic 96 Protocol
preset at 65-70°C 4. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000) 实验步骤 Tissue Disruption Manual disruption: To prepare samples, collect plant sample in a 30 ml mortar and freeze
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