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文献和实验Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays
The ability to profile the miRNome (global microRNA/miRNA expression levels) accurately has become essential for multiple aspects of biological research. However, this process is complicated by the short length of the mature
MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells
The Mouse P19 cell line was derived from an embryonal carcinoma. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles
提供miRNA定量检测启动装，一个试剂盒里面包含逆转录、内参引、阳性对照、real-time PCR扩增所有的组分，您很快就可以学会定量分析microRNA的表达量了。 实验进行到这里，只是在进行miRNA量变的研究，更进一步可以升华到某些特定miRNA功能的探索，采用类似RNAi沉默技术，miRNA同样可以被沉默或者高表达，QIAGEN提供miRNA Inhibitors和miRNA Synthesis 产品来分别完成，详细的实验方案请见下回分解。 microRNA 研究实验导航—功能分析 找到差异
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