CAGs-GFP-T2A-Luciferase Dual R

Dual- and Triple-Color Fluorospot

cytokines. The ability of CD4 and CD8 T cells to respond to antigen with a combination of, e.g., IFN-γ, IL-2, and TNF-α, rather than only one of the cytokines, has been associated with enhanced protective immunity in viral, ­bacterial, as well as parasitic

Two‐Hybrid Dual Bait System

for screening of these dual baits possess four separate reporter genes. A plasmid expressing an activation domain?fused protein (prey), which can be either a defined protein interactor or a cDNA library, is also expressed to allow dual hybrid?mediated

Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers

via hybridization or nuclease activity. Fluorescence resonance energy transfer (FRET) is the physical mechanism that is most often cited to describe how quenching occurs. We have found that many dual-labeled probes have enhanced quenching