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文献和实验. 5 ml 1 M Tris-HCl, pH 8.0 2 ml 0.5 M EDTA 2 ml Triton X-100 (Sigma X-100) ddH2O to 100 ml TTE: 10 mM Tris-HCl, pH 8.0, 0.5% Triton X-100, and 0.1 mM EDTA in double distilled water. 500 ul 1 M Tris-HCl, pH 8.0 250 ul Triton X
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
. It may also provide a valuable tool in the discovery of agents that modulate oxidative stress in various pathologies. RedoxSensor Red CC-1 Stain RedoxSensor Red CC-1 (2,3,4,5,6-pentafluorotetramethyldihydrorosamine, R14060; Figure 12.19) stain is a unique probe
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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