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文献和实验Cell cycle analysis of Escherichia coli cells
Cell cycle analysis of Escherichia coli cells C period = the time for a round of chromosome replication D period = the time between the end of a round of chromosome replication and cell division Determination of initiation age
Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis
Synchronized cells have been invaluable in many kinds of cell cycle and cell proliferation studies. Butyrate induces cell cycle arrest and apoptosis in Madin Darby Bovine Kidney (MDBK) cells. We explore the possibility of using butyrate
cold' 70% ethanol to 1x106 cells while vortexing the cell pellet gently. Fix for at least 30 min. at 4° C. Spin at 2000 rpm for 5 min. Once in 70% ethanol, samples may be kept for up to 2 weeks. Discard supernatant and resuspend cells
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