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文献和实验After mixing, remove the 1L bag from the mixer and drain 150 ml of Buffer 1 into the bag. [Note: Handle the 1L bag very gently, to not disrupt the bead/cell complexes.] Place the 1L bag directly on the DynaMag™ CTS™. Adjust the magnet to a 60° angle. 8)
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
实验试剂 Buffer: Phosphate buffered saline with 0.1% bovine serum albumin and 2 mM EDTA, pH 7.4 (PBS w/0.1% BSA). Magnet (DynaMag™): See www.invitrogen. com/magnets for magnet recommendations. Culture medium: Advanced
Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
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